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มิญชวิทยา Common laboratory stainsFungi—black
สีย้อม | การใช้งาน | นิวเคลียส | ไซโตพลาสซึม | เซลล์เม็ดเลือดแดง (RBC) | เส้นใยคอลลาเจน | Specifically stains |
---|---|---|---|---|---|---|
Haematoxylin | สีย้อมทั่วไป ใช้คู่กับ Eosin | Blue | N/A | N/A | N/A | กรดนิวคลีอิค—น้ำเงิน Blue eER (ergastoplasm) —blue |
Eosin | สีย้อมทั่วไป ใช้คู่กับ Haematoxylin | N/A | ชมพู | ส้ม/แดง | ชมพู | เส้นใยอิลาสติก—ชมพู เส้นใยเรติคูลา—ชมพู |
Toluidine blue | General staining | Blue | Blue | Blue | Blue | Mast cells granules—purple |
Masson's trichrome stain | Connective tissue | Black | Red/pink | Red | Blue/green | Cartilage—blue/green Muscle fibers—red |
Mallory's trichrome stain | Connective tissue | Red | Pale red | Orange | Deep blue | Keratin—orange Cartilage—blueBone matrix—deep blueMuscle fibers—red |
Weigert's elastic stain | Elastic fibers | Blue/black | N/A | N/A | N/A | Elastic fibers—blue/black |
Heidenhains'azan trichrome stain | Distinguishing cells from extracellular components | Red/purple | Pink | Red | Blue | Muscle fibers—red Cartilage—blueBone matrix—blue |
Silver stain | Reticular fibers, nerve fibers, fungi | N/A | N/A | N/A | Reticular fibers—brown/black Nerve fibers—brown/black | |
Wright's stain | Blood cells | Bluish/purple | Bluish/gray | Red/pink | N/A | Neutrophil granules—purple/pink Eosinophil granules—bright red/orangeBasophil granules—deep purple/violetPlatelet granules—red/purple |
Orcein stain | Elastic fibres | Deep blue [or crazy red] | N/A | Bright red | Pink | Elastic fibres—dark brown Mast cells granules—purpleSmooth muscle—light blue |
Periodic acid-Schiff stain (PAS) | Basement membrane, localizing carbohydrates | Blue | N/A | N/A | Pink | Glycogen and other carbohydrates—magenta |
Table sourced from Michael H. Ross, Wojciech Pawlina, (2006). Histology: A Text and Atlas. Hagerstown, MD: Lippincott Williams & Wilkins. ISBN 0-7817-5056-3.CS1 maint: Multiple names: authors list (link)
The Nissl method and Golgi's method are useful in identifying neurons.
Alternative techniques include cryosection. The tissue is frozen and cut using a cryostat. Tissue staining methods are similar to those of wax sections. Plastic embedding is commonly used in the preparation of material for electron microscopy. Tissues are embedded in epoxy resin. Very thin sections (less than 0.1 micrometer) are cut using diamond or glass knives. The sections are stained with electron dense stains (uranium and lead) so that they can possibly be seen with the electron microscope.
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มิญชวิทยา Common laboratory stainsใกล้เคียง
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WikiPedia: มิญชวิทยา http://www.hgv.org.au http://www.histology-world.com/glossary/glossary1.... http://www.histology-world.com/photoalbum/ http://www.ihcworld.com/protocol_database.htm http://www.immunoportal.com http://stedmans.com/ http://www.visualhistology.com/Visual_Histology_At... http://www.kumc.edu/instruction/medicine/anatomy/h... http://www.siumed.edu/~dking2/index.htm http://rirs3.royin.go.th/coinages/webcoinage.php