Common laboratory stains ของ มิญชวิทยา

Fungi—black

สีย้อมการใช้งานนิวเคลียสไซโตพลาสซึมเซลล์เม็ดเลือดแดง (RBC)เส้นใยคอลลาเจนSpecifically stains
Haematoxylinสีย้อมทั่วไป ใช้คู่กับ EosinBlueN/AN/AN/Aกรดนิวคลีอิค—น้ำเงิน

Blue eER (ergastoplasm) —blue

Eosinสีย้อมทั่วไป ใช้คู่กับ HaematoxylinN/Aชมพูส้ม/แดงชมพูเส้นใยอิลาสติก—ชมพู

เส้นใยเรติคูลา—ชมพู

Toluidine blueGeneral staining BlueBlueBlueBlueMast cells granules—purple
Masson's trichrome stainConnective tissueBlackRed/pinkRedBlue/greenCartilage—blue/green

Muscle fibers—red

Mallory's trichrome stainConnective tissueRedPale redOrangeDeep blueKeratin—orange

Cartilage—blueBone matrix—deep blueMuscle fibers—red

Weigert's elastic stainElastic fibersBlue/blackN/AN/AN/AElastic fibers—blue/black
Heidenhains'azan trichrome stainDistinguishing cells from extracellular componentsRed/purplePinkRedBlueMuscle fibers—red

Cartilage—blueBone matrix—blue

Silver stainReticular fibers, nerve fibers, fungiN/AN/AN/AReticular fibers—brown/black

Nerve fibers—brown/black

Wright's stainBlood cellsBluish/purpleBluish/grayRed/pinkN/ANeutrophil granules—purple/pink

Eosinophil granules—bright red/orangeBasophil granules—deep purple/violetPlatelet granules—red/purple

Orcein stainElastic fibresDeep blue [or crazy red]N/ABright redPinkElastic fibres—dark brown

Mast cells granules—purpleSmooth muscle—light blue

Periodic acid-Schiff stain (PAS) Basement membrane, localizing carbohydratesBlueN/AN/APinkGlycogen and other carbohydrates—magenta

Table sourced from Michael H. Ross, Wojciech Pawlina, (2006). Histology: A Text and Atlas. Hagerstown, MD: Lippincott Williams & Wilkins. ISBN 0-7817-5056-3.CS1 maint: Multiple names: authors list (link)

The Nissl method and Golgi's method are useful in identifying neurons.

Alternative techniques

Alternative techniques include cryosection. The tissue is frozen and cut using a cryostat. Tissue staining methods are similar to those of wax sections. Plastic embedding is commonly used in the preparation of material for electron microscopy. Tissues are embedded in epoxy resin. Very thin sections (less than 0.1 micrometer) are cut using diamond or glass knives. The sections are stained with electron dense stains (uranium and lead) so that they can possibly be seen with the electron microscope.